TRIzol for plasmid DNA isolation

Plasmid isolation. Fatemeh Mehrpooyan*

Improved alkaline lysis method for rapid isolation of plasmid DNA.

Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...

Rapid plasmid DNA isolation from mucoid gram-negative bacteria.

Exopolysaccharides interfere with the isolation and characterization of plasmid DNA from gram-negative bacteria. To repress capsular polysaccharide production, bacteria were cultured in medium containing bismuth nitrate and sodium salicylate. Rapid removal of other contaminating bacterial surface components was achieved by mild acidic zwitterionic detergent extraction. After treatment, bacteria...

Trizol Tm : a New Reagent for Optimal Single - Step Isolation of Rna

Piotr Chomczynski Department of Internal Medicine School of Medicine University of Cincinnati Cincinnati, Ohio 45267 Progress in many fields of basic and clinical research depends on the availability of high-quality RNA. Of the many methods for isolating RNA (reviewed in 1 and 2), a single-step RNA isolation method based on guanidine isothiocyanate/phenol/chloroform extraction (3) has been grow...

Isolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage.

A systems approach is being applied in many areas of the biological sciences, particularly in cancer research. The coordinated, simultaneous extraction of DNA, RNA, and proteins from a single sample is crucial for accurate correlations between genomic aberrations and their consequences on the transcriptome and proteome. We present an approach to extract and completely solubilize up to 98% of th...